Brain Heart Infusion Agar w/ 3.0% Agar

Intended Use

Brain Heart Infusion Agar w/ 3.0% Agar (DM1038) is recommended for cultivation of microorganisms when high concentration of Agar gel is desired.

Product Summary and Explanation

Brain Heart Infusion Agar with 3% Agar is a general purpose culture medium used for primary isolation of aerobic bacteria from clinical specimens, ¹ and is highly nutritious medium which can support luxuriant growth of wide variety of microorganisms. Using hard (3%) agar gel it is used for cultivation of fastidious microorganisms.

Principles of the Procedure

Brain Heart Infusion Agar w/ 3.0% Agar contains proteose peptone and infusions (Calf brain Beef heart) which provides carbon, nitrogen, vitamins, amino acids and other essential growth factors. Dextrose is a source of carbon and energy. Sodium chloride maintains the osmotic equilibrium of the medium. Disodium phosphate acts as a buffering agent.

Formula / Liter

Precautions

1. For Laboratory Use only.
2. IRRITANT. Irritating to eyes, respiratory system, and skin.

Directions

1. Suspend 67 grams of the medium in one litre distilled water.
2. Heat to boiling to dissolve the medium completely.
3. Dispense as desired.
4. Autoclave at 121°C, 15 psi pressure, for 15 minutes / validated cycle.

Quality Control Specifications

Expected Cultural Response: Cultural characteristics observed after an incubation at 35- 37°C for 18-24 hours.

The organisms listed are the minimum that should be used for quality control testing.

Test Procedure

Refer to appropriate references for standard test procedures.

Results

Refer to appropriate references for interpretation of results.

Storage

Store the sealed bottle containing the dehydrated medium at 10- 30°C. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light.

Expiration

Refer to the expiration date stamped on the container. The dehydrated medium should be discarded if not free flowing, or if the appearance has changed from the original color. Expiry applies to medium in its intact container when stored as directed.

Limitations of the Procedure

1. For identification, organisms must be in pure culture. Morphological, biochemical and/or serological tests should be performed for final identification.
2. Consult appropriate texts for detailed information and recommended procedures.